Determination of Total Ginsenosides in Panax Capsule using Spectrophotometry and High Performance Liquid Chromatography (HPLC)

ching-lee-fungProject Title: Determination of Total Ginsenosides in Panax Capsule using Spectrophotometry and High Performance Liquid Chromatography (HPLC)

Research ID: 4/2016/10

Principal Researcher: Ching Lee Fung

Co-Researchers: n/a

Project Start Date: 12/1/2016

Duration of Project: 24 months

Research Summary: Ginseng (panax genus plant) has been used as an herbal medicine in Asia for over two thousand years for its various health benefits as antioxidant, anti-carcinogenic, anti-inflammatory, anti-hypertensive and anti-diabetic. The pharmacologically active compounds behind the claims of ginseng’s efficacy are ginsenosides. Most of the ginseng products (ginseng root, body, leaves) grounded to powder and were packaged in tablets or capsules form for ease of consumption. Ginseng can be purchased as an extract and is often standardized to contain between 5% to 25% ginsenosides. However, products are often standardized to low amount of active components. Most are found diluted with excipients, blended with adulterants, or totally devoid of active constituents (ginsenosides). Therefore, qualitative and quantitative analytical techniques for the analysis of ginsenosides are in demand to ensure quality control in ginseng processing. Thus, the main objective of this research is to quantify the total ginsenosides content per capsule which are obtained from a Traditional Chinese Medicine (TCM) store around Sarawak. Generally, the method will involve the extraction of active ingredient ginsenosides by a series of solvent extractions and follow by qualitative determination using UV Vis spectrometer. For quantification of total gensinosides, ginseng will be analyzed by high performance liquid chromatography (HPLC). The method is applied to a panax ginseng (Korean Ginseng) root capsules and the total ginsenosides are confirmed. The findings from this research serves as a monitoring steps to protect consumers from further exploitation.