Preliminary Development of Meat Admixture Detection of Meat spp Multiplex Real Time PCR enhanced Taqman Probe

Nurhayati-Binti--SajaliProject Title:  Preliminary Development of Meat Admixture Detection of Meat spp Multiplex Real Time PCR enhanced Taqman Probe

Research ID:  4/2016/06

Principal Researcher:  Nurhayatie binti Sajali

Co-Researchers:  Dr. Mohd Zahid Abidin

Project Start Date:  12/1/2016

Duration of Project:  24 months

Research Summary:  Meat adulteration incidences are escalating with global demand for meat based products. In order to constantly provide safe and hygienic food, authenticity in food ingredients are pivotal. Development of rapid, sensitive and specific method for identification of meat origin in processed food are essential to enhance the chances for detection of food adulteration. Hence, multiple target with concurrent quantification at one time may reduce cost and time for authenticating different meat species. Based on common primer multiplex PCR (CP-M-PCR) model for detecting ruminant and avian species developed in a previous study (Hanapi et al., 2015), modification of approach with the use of Real Time PCR technique using multiple DNA probes will be assessed in the current study. This model could serve as a validation for testing against food adulteration on various processed food in the future.